Animal Cell Culture Bioreactors

Requirements for a bioreactor for animal cell culture 1 well controlled environment t ph do nutrients and wastes 2 supply of nutrients 3 gentle mixing avoid shear damage to cells 4 gentle aeration add oxygen slowly to the culture medium but avoid the formation of large bubbles which can damage cells on contact.

Animal cell culture bioreactors.

This article examines the underlying mechanisms involved in bubble associated cell damage and the methods available for controlling such damage. However despite this sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen especially on a large scale. It addresses engineering aspects such as mass transfer instrumentation and control ensuring successful design and operation of animal cell bioreactors. Animal cell culture control for bioprocess engineering presents the design fabrication and control of a new type of bioreactor meant especially for animal cell line culture.

In one case a bioreactor is a vessel in which a chemical process is carried out which involves organisms or biochemically active substances derived from such organisms. This process can either be aerobic or anaerobic these bioreactors are commonly cylindrical ranging in size from litres. Animal cell bioreactors provides an introduction to the underlying principles and strategies in the in vitro cell culture biotechnology. There are several types of bioreactors including stirred tank airlift hollow fiber and rotary cell culture system rccs d.

A bioreactor refers to any manufactured device or system that supports a biologically active environment. One bacterial spore or vegetative bacterial cell is all it takes to overgrow an animal cell culture. Morphological and biochemical features of two major mammalian cell groups. Bioreactors play a key role in the field of biologics where they are used for the production of recombinant therapeutic proteins by large scale cultivation of animal cells.

The gas sparging of culture broth causes damage to suspended animal cells. Animal cell culture control for bioprocess engineering presents the design fabrication and control of a new type of bioreactor meant especially for animal cell line culture. The new bioreactor called the see saw bioreactor is ideal for the growth of cells with a sensitive membrane. Microcarriers with adherent cells which were in principle an adaptation of homogeneous culture systems used for.

Animal cell culture bioreactors initially employed stirred tanks containing. Cell cultures are particularly susceptible to biological contamination.